Genetic immunization is a promising strategy for inducing protective immune responses against a great variety of bacterial pathogens infections. Compared with the conventional live or subunit vaccines, DNA vaccine has several potential advantages such as being easier to manufacture, having greater stability, and conferring potential safety 24, 25. Interleukin-1 beta (IL-1?) is a glycoprotein produced by activated macrophages and blood monocytes 26. The active IL-1? can induce the expression of immune-related molecules and promote a cascade of immune responses 27, 28. Additionally, IL-1? can be used as a molecular adjuvant to augment the immunogenicity and to increase the protective efficacy of DNA vaccines 29-31. In this study, we constructed a recombinant plasmid pVAX1-PLO using the plo gene of T. pyogenes. The immunogenicity of pVAX1-PLO and a recombinant plasmid pcDNA3.1/V5-fIL-1? encoding M. berezovskii IL-1? were investigated in mouse model. We found that pVAX1-PLO induced humoral and cellular immune responses and protected mice from T. pyogenes infection. The plasmid pcDNA3.1/V5-fIL-1? enhanced the immunogenicity of pVAX1-PLO, therefore provided a possibility to control T. pyogenes infections and facilitating the development of efficacious bacterial gene vaccines against T. pyogenes related diseases.